Surfactants are widely applied as detergents for cleaning oil-based stains. To better understand surfactants-oil interactions, especially in the case of oil film, an experiment is done to study the detergent-water mixture droplet impact on an oil film on top of a water pool. For comparison, droplets of pure water, as well as a detergent-water mixture, are produced through a syringe-needle device. Oil films of controlled thickness are injected into the water in a square transparent container. During each test, a droplet impacts the surface of an oil-water pool and the whole process is recorded by a Photron FASTCAM Mini AX100 highspeed camera. The rebound height after each droplet impact is determined through image processing using MATLAB. The analysis of temporal evaluations of the rebound heights shows that the rebound process takes place earlier for the detergent droplet test than for pure water droplet tests. Besides, the maximum rebound is higher for droplets with larger diameters, and the existence of surfactant reduces the maximum rebound in comparison with pure water droplets. Finally, the maximum rebound is linearly correlated with the Weber number, while the data for surfactant droplets and pure water droplets lies on two different lines.